Difference between revisions of "Part:BBa K1465205"
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===iGEM2017 SZU-China=== | ===iGEM2017 SZU-China=== | ||
We add the RBS of Bacillus subtilis and a segment of signal peptide at the upstream of cds of Carboxysomal Carbonic Anhydrase(csoS3) from Halothiobacillus neapolitanus, in order to achieve the expression and secretion of this part in the B.subtilis WB800. The construction of the shuttle expression-secretion vector_CsoS3 is shown as follow in figure 1. | We add the RBS of Bacillus subtilis and a segment of signal peptide at the upstream of cds of Carboxysomal Carbonic Anhydrase(csoS3) from Halothiobacillus neapolitanus, in order to achieve the expression and secretion of this part in the B.subtilis WB800. The construction of the shuttle expression-secretion vector_CsoS3 is shown as follow in figure 1. | ||
| + | <div> | ||
| + | <center><html><img src='https://static.igem.org/mediawiki/parts/thumb/4/42/CsoS3_2.png/302px-CsoS3_2.png' > | ||
| + | <center>Fig.19 Construction of the shuttle expression-secretion vector_CsoS3.</center> | ||
| + | </html></center> | ||
| + | |||
| + | </div> | ||
| + | The new part (BBa_K2232022) was constructed into shuttle expression-secretion vector pP43NMK, and 1% Agarose Gel Electrophoresis of the plasmid verified the correct construction in figure 2. | ||
<div> | <div> | ||
<center><html><img src='https://static.igem.org/mediawiki/parts/thumb/6/68/CsoS3_1.png/179px-CsoS3_1.png' ></html></center> | <center><html><img src='https://static.igem.org/mediawiki/parts/thumb/6/68/CsoS3_1.png/179px-CsoS3_1.png' ></html></center> | ||
</div> | </div> | ||
Revision as of 17:44, 1 November 2017
Carbonic anhydrase (csoS3) of the carboxysome of Halothiobacillus neapolitanus
The carbonic anhydrase from Halothiobacillus neapolitanus converts incoming hydrogen carbonate into carbon dioxide inside the carboxysome. This step is essential for the CO2 fixation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 166
- 1000COMPATIBLE WITH RFC[1000]
User Reviews
james_zhang
In 2017 SZU-China iGEM team changed the sequence of original part(BBa_K1465205) and the new part BBa_K2232022 was submited.
iGEM2017 SZU-China
We add the RBS of Bacillus subtilis and a segment of signal peptide at the upstream of cds of Carboxysomal Carbonic Anhydrase(csoS3) from Halothiobacillus neapolitanus, in order to achieve the expression and secretion of this part in the B.subtilis WB800. The construction of the shuttle expression-secretion vector_CsoS3 is shown as follow in figure 1.
The new part (BBa_K2232022) was constructed into shuttle expression-secretion vector pP43NMK, and 1% Agarose Gel Electrophoresis of the plasmid verified the correct construction in figure 2.
