Difference between revisions of "Part:BBa K2384008"
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required for optimal responses to high concentrations. In order to elucidate these processes, chemotactic responses were assessed for strains
 
required for optimal responses to high concentrations. In order to elucidate these processes, chemotactic responses were assessed for strains
 
expressing methylation-defective mutations in the asparagine receptor, McpB.
 
expressing methylation-defective mutations in the asparagine receptor, McpB.
 +
<b>Optimization Report</b>
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Sequence optimization information:
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Expression System: <i>Bacillus megaterium</i>
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Gene Length:1986(bp)
 
<br/><br/>
 
<br/><br/>
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 +
Show the relative codon used distribution
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 +
 +
<table><tr>
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<th>[[Image:T--FAFU-CHINA-1mcpb.png|thumb|400px|'''Figure 4 ''':Codon Used Distribution after optimization. ]]</th>
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</tr></table>
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 +
<br/><br/>
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<table><tr>
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<th>[[Image:T--FAFU-CHINA-2mcpb.png|thumb|400px|'''Figure  5''':GC Content after optimization. ]]</th>
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</tr></table>
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<table><tr>
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<th>[[Image:T--FAFU-CHINA-dnamcpb.png|thumb|400px|'''Figure 3''': Digested with EcoRI//. ]]</th>
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</tr></table>
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Revision as of 17:27, 1 November 2017


McpB

Chemotaxis is the process by which motile bacteria sense their environment and move towards more favorable surroundings. Environmental ligands are sensed upon binding to transmembrane receptors called methyl-accepting chemotaxis proteins (MCPs).


Usage and Biology



Asparagine chemotaxis in Bacillus subtilis appears to involve two partially redundant adaptation mechanisms: a receptor methylation-independent process that operates at low attractant concentrations and a receptor methylation-dependent process that is required for optimal responses to high concentrations. In order to elucidate these processes, chemotactic responses were assessed for strains expressing methylation-defective mutations in the asparagine receptor, McpB. Optimization Report Sequence optimization information:

Expression System: Bacillus megaterium

Gene Length:1986(bp)

Show the relative codon used distribution


Figure 4 :Codon Used Distribution after optimization.



Figure 5:GC Content after optimization.
Figure 3: Digested with EcoRI//.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]