Difference between revisions of "Part:BBa K2348012"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The part as pH regulated mNeonGreen expression device. Data of expression is shown in the alx Promoter and riboswitch part [https://parts.igem.org/Part:BBa_K2348000 BBa_K2348000]. | + | The part is used as pH regulated mNeonGreen expression device. Data of expression is shown in the alx Promoter and riboswitch part [https://parts.igem.org/Part:BBa_K2348000 BBa_K2348000]. |
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Latest revision as of 16:54, 1 November 2017
alx promoter and riboswitch + mNeonGreen
This part actually is a composite part containing the alx promoter and riboswitch BBa_K2348000 and mNeonGreen BBa_K1761003. But in addition, between riboswitch and mNeonGreen a TEV-site and a f-degron are inserted. Also, mNeonGreen ins 6xHis-taged and the parts ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and F-degron allow a fast and controllable degradation of mNeonGreen wich, in combination with the fast maturation time of mNeonGreen, lead to a fast colour change when used together with our other pH-sensitive part BBa_K2348011
Usage and Biology
The part is used as pH regulated mNeonGreen expression device. Data of expression is shown in the alx Promoter and riboswitch part BBa_K2348000.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 312