Difference between revisions of "Part:BBa K2384004"
| Line 5: | Line 5: | ||
DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically . | DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically . | ||
| − | Our sequence optimization is based on the sequence provided by Peking University's IGEM | + | Our sequence optimization is based on the sequence provided by Peking University's IGEM Wiki in 2010.It is more suitable for expression in Bacillus |
Revision as of 18:33, 1 November 2017
DsbA+MBP(Hg)
DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically .
Our sequence optimization is based on the sequence provided by Peking University's IGEM Wiki in 2010.It is more suitable for expression in Bacillus
Usage and Biology
Optimization Report
1.Codon Used Adjustment The best value is 1 for sequence optimization.
1.png)
2.png)
2.Codon Used Distribution
Show the relative codon used distribution
3.png)
4.png)
3.GC Content: The comparison of GC content between original sequence and optimized sequence
5.png)
6.png)
.png)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 255
Illegal BglII site found at 267
Illegal BglII site found at 711 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]