Difference between revisions of "Part:BBa K2348012"

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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
<a href="https://static.igem.org/mediawiki/2017/2/27/Alx_part_Graz.jpeg"><img src="Alx_part_Graz.jpeg/799px-Alx_part_Graz.jpegg" alt="Bild" /></a>
 
 
 
https://static.igem.org/mediawiki/2017/thumb/2/27/Alx_part_Graz.jpeg/799px-Alx_part_Graz.jpeg
 
https://static.igem.org/mediawiki/2017/thumb/2/27/Alx_part_Graz.jpeg/799px-Alx_part_Graz.jpeg
  

Revision as of 15:55, 1 November 2017


alx promoter and riboswitch + mNeonGreen
This part actually is a composite part containing the alx promoter and riboswitch BBa_K2348000 and mNeonGreen BBa_K1761003. But in addition, between riboswitch and mNeonGreen a TEV-site and a f-degron are inserted. Also, mNeonGreen ins 6xHis-taged and the parts ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and f-degron allow a fast and controllable degradation of mNeonGreen and in combination with the fast maturation time of mNeonGreen allow a fast cooler change when used together with our other pH-sensitive part BBa_K2348011


Sequence and Features 799px-Alx_part_Graz.jpeg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 312