Difference between revisions of "Part:BBa K2524002"
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| − | The Factor C-hCG fusion protein can allow for the detection of gram-negative bacteria in a solution. With hCG fused to the end of Factor C that is immobilized in a solution, it can be used as a detection signal for the activation of Factor C. When LPS is introduced into the solution the hCG will be cut and be released into the solution. When a pregnancy test is inserted the pregnancy test will produce a positive result. The hCG is fused to the end of the Light chain of the Factor C. | + | The Factor C-hCG fusion protein can allow for the detection of gram-negative bacteria in a solution. With hCG fused to the end of Factor C that is immobilized in a solution, <br> it can be used as a detection signal for the activation of Factor C. When LPS is introduced into the solution the hCG will be cut and be released into the solution. When a pregnancy test is inserted the pregnancy test will produce a positive result. The hCG is fused to the end of the Light chain of the Factor C. |
<!-- Factor C is an enzyme produced in the Horseshoe crab. It is apart of their immune system. The Factor C protein is the first in a cascade that causes their blood to clot in the presence of lipopolysaccharide (LPS) located on the outer membrane of gram-negative bacteria. When Factor C is in the presence of LPS it undergoes autocatalysis. This is very helpful upon detection of minuscule concentrations of gram-negative bacteria. Factor C is a glycoprotein with a molecular mass of 123 kD. It has an Heavy chain (80kD) and Light chain (43 kD). In the presence of LPS, factor C is activated and undergoes autocatalysis and cleaves the Phe-Ile bond. This autocatalysis results in the formation of two new fragments: B chain (34kD) and A chain (8.5 kD). | <!-- Factor C is an enzyme produced in the Horseshoe crab. It is apart of their immune system. The Factor C protein is the first in a cascade that causes their blood to clot in the presence of lipopolysaccharide (LPS) located on the outer membrane of gram-negative bacteria. When Factor C is in the presence of LPS it undergoes autocatalysis. This is very helpful upon detection of minuscule concentrations of gram-negative bacteria. Factor C is a glycoprotein with a molecular mass of 123 kD. It has an Heavy chain (80kD) and Light chain (43 kD). In the presence of LPS, factor C is activated and undergoes autocatalysis and cleaves the Phe-Ile bond. This autocatalysis results in the formation of two new fragments: B chain (34kD) and A chain (8.5 kD). | ||
Revision as of 15:54, 1 November 2017
Limulus Clotting Factor C-hCG fusion protein
The Factor C-hCG fusion protein can allow for the detection of gram-negative bacteria in a solution. With hCG fused to the end of Factor C that is immobilized in a solution,
it can be used as a detection signal for the activation of Factor C. When LPS is introduced into the solution the hCG will be cut and be released into the solution. When a pregnancy test is inserted the pregnancy test will produce a positive result. The hCG is fused to the end of the Light chain of the Factor C.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]