Difference between revisions of "Part:BBa K2232020"

Revision as of 02:34, 2 November 2017

PsspB-gerAa

This part expresses in B.subsilis to increase the germinability of the spore.

Usage and Biology

This part，PsspB-gerAa (BBa_K2232020)，consisting mainly of the PsspB promoter followed by the first 303bp of the gerA gene, plays the role as Responder in the germination of spores. The gerA from Bacillus subtilis encodes the spore’s germinant receptor for L-alanine. Overexpression of this gene will increase markedly the rate of germination of B. subtilis spores even at low L-alanine concentration. The promoter for the B.subtilis sspB gene (PsspB) is stronger than the endogenous gerA promoter, and only switch on the transcription after the initiation of sporulation. Since B.subtilis exhibits accurate and efficient homologous recombination, a single cross-over event between the gerAa region in the BioBrick and the endogenous gerA sequence inserts the PsspB promoter upstream of the gerA cds, which increases the germination rate of Bacillus subtilis endospores eventually.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 7
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 94
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 409

References

This part created by 2017 SZU-China iGEM team basing on the original part: BBa_K911008

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 ===Usage and Biology===
-The gerA operon from Bacillus subtilis encodes the spore’s germinant receptor for L-alanine. Overexpression of the tricistronic gerA operon will increase markedly the rates of germination of Bacillus subtilis spores with L-alanine,in particular at low L-alanine concentrations.
+This part，PsspB-gerAa (BBa_K2232020)，consisting mainly of the PsspB promoter followed by the first 303bp of the gerA gene, plays the role as Responder in the germination of spores.
-The promoter for the B.subtilis sspB gene (PsspB) is more active than the endogenous gerAa promoter. It is also active during germination.
+The gerA from Bacillus subtilis encodes the spore’s germinant receptor for L-alanine. Overexpression of this gene will increase markedly the rate of germination of B. subtilis spores even at low L-alanine concentration.
-This device consists mainly of the sspB promoter followed by the first 303bp of the gerA operon (first 303bp of the gerAa gene). Since B.subtilis exhibits accurate and efficient homologous recombination, a single cross-over event between the gerAa region in the BioBrick and the endogenous gerA sequence inserts the sspB promoter upstream of the spoVA operon,which promote the germination of Bacillus subtilis.
+The promoter for the B.subtilis sspB gene (PsspB) is stronger than the endogenous gerA promoter, and only switch on the transcription after the initiation of sporulation.
+Since B.subtilis exhibits accurate and efficient homologous recombination, a single cross-over event between the gerAa region in the BioBrick and the endogenous gerA sequence inserts the PsspB promoter upstream of the gerA cds, which increases the germination rate of Bacillus subtilis endospores eventually.
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