Difference between revisions of "Part:BBa K2243010"
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We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop. | We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop. | ||
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+ | <img src="https://static.igem.org/mediawiki/2017/f/fc/Peking_flipflop_fig_5.svg" height="500" width="600"/> | ||
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+ | </body> | ||
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+ | Figure 2. The standard testing system used to characterize the recombinases. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 16:06, 1 November 2017
TP901-1 attB_J23119_TP901-1 attP
The constitutive promoter J23119 is between Bxb1 gp35 specific sites and will be inverted by the Bxb1 gp35 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase bxb1 gp35 characterization.
We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.
Figure 2. The standard testing system used to characterize the recombinases.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 105
Illegal NheI site found at 128 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 188
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]