Difference between revisions of "Part:BBa K2505035:Design"
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==Design Notes== | ==Design Notes== | ||
The gene <i>traI</i>(K34G, Q63G) is derived from <i>Agrobacterium tumefaciens</i> and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in <i>E. coli</i>. | The gene <i>traI</i>(K34G, Q63G) is derived from <i>Agrobacterium tumefaciens</i> and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in <i>E. coli</i>. | ||
| + | |||
| + | The mutation was introduced wild type <i>traI</i>(BBa_K553001). | ||
| + | |||
| + | The DNA sequences of <i>traI</i> (K34G, Q63G) is optimized for expressing in <i>E. coli</i> considering the codon usage | ||
Latest revision as of 13:56, 1 November 2017
traI (K34G, Q63G)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene traI(K34G, Q63G) is derived from Agrobacterium tumefaciens and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in E. coli.
The mutation was introduced wild type traI(BBa_K553001).
The DNA sequences of traI (K34G, Q63G) is optimized for expressing in E. coli considering the codon usage
Source
Mutation was newly introduced on the BBa_K553001 by PCR
References
[1] Pavan Kumar Reddy Kambam, Daniel J. Sayut, Yan Niu, Dawn T. Eriksen, Lianhong Sun (2008) Directed evolution of LuxI for enhanced OHHL production. Biotechnology and Bioengineering Volume 101, Issue 2 1 October 2008 Pages 263-272
[2] MATTHEW R. PARSEK, DALE L. VAL, BRIAN L. HANZELKA, JOHN E. CRONAN, E. P. GREENBERG (1999) Acyl homoserine-lactone quorum-sensing signal generation. Proc. Natl. Acad. Sci. USA Vol. 96, pp. 4360-4365, April 1999 Biochemistry