Difference between revisions of "Part:BBa K2398563:Design"
 
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
todo
+
This part was modified from the beta-glucuronidase coding sequence of <i>E. coli</i>, obtained from AddGene (#61156).
  
 
===References===
 
===References===

Latest revision as of 13:45, 1 November 2017


Beta-Glucuronidase_D508G


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 506
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created from the wildtype E.coli beta-glucuronidase. One or more amino acid substitutions were introduced. It was used for protein expression under a lac inducible T7 promoter.

Source

This part was modified from the beta-glucuronidase coding sequence of E. coli, obtained from AddGene (#61156).

References