Difference between revisions of "Part:BBa K2398505:Design"
 
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<partinfo>BBa_K2398505 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2398505 SequenceAndFeatures</partinfo>
  
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This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3. <br><br>
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This part is the coding sequence of the E. coli beta-lactamase. <br><br>
 
In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
 
In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
  

Latest revision as of 15:48, 1 November 2017


beta_lactamase_Y103D_G216C_P217F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This part is the coding sequence of the E. coli beta-lactamase.

In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).

Design Notes

compatibla with RFC10


Source

genomic with modifications

References