Difference between revisions of "Part:BBa K2201010"

Latest revision as of 13:12, 1 November 2017

Construct comprised of PtNTT2, lacO and Cas9

Usage and Biology

This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of PtNTT2, please view BBa_K2201004. A characterization of the lacO operon and Cas9 can be found here: BBa_K2201020 and BBa_K2201023.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 3356
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3356
Illegal NheI site found at 3115
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3356
Illegal BamHI site found at 5394
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 3356
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 3356
1000
COMPATIBLE WITH RFC[1000]

@@ Line 13: / Line 13: @@
-<span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K2201010 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
-<partinfo>BBa_K2201010 parameters</partinfo>
-<!-- -->
-__NOTOC__
-<partinfo>BBa_K2201010 short</partinfo>
-Construct comprised of PtNTT2, lacO and Cas9
-===Usage and Biology===
-This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of <i>Pt</i>NTT2,
-please view <a href="https://parts.igem.org/Part:BBa_K2201004"> BBa_K2201004</a>. A characterization of the lacO operon and Cas9 can be found here: <a href="https://parts.igem.org/Part:BBa_K2201020">BBa_K2201020</a> and <a href="https://parts.igem.org/Part:BBa_K2201023">BBa_K2201023</a>.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2201010 SequenceAndFeatures</partinfo>