Difference between revisions of "Part:BBa K2398556"

Revision as of 14:12, 3 October 2020

T7 Promoter

T7 promoter for protein Expression. The promoter was used by the iGEM Team Heidelberg in the context of phage assisted continous evolution (PACE).

Usage and Biology

Contribution: New documentation from Evry_Paris-Saclay 2020

This part is the strong promoter from T7 bacteriophage (taatacgactcactata) with GGG of 3' end. The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GGG is one of the best +1, +2 and +3 base combinations at the transcription initiation for enhanced promoter strength [1].

[1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 T7 promoter for protein Expression. The promoter was used by the iGEM Team Heidelberg in the context of phage assisted continous evolution (PACE).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+===Contribution: New documentation from Evry_Paris-Saclay 2020===
+This part is the strong promoter from T7 bacteriophage (taatacgactcactata) with GGG of 3' end.
+The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GGG is one of the best +1, +2 and +3 base combinations at the transcription initiation for enhanced promoter strength [1].
+[1] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.
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