Difference between revisions of "Part:BBa K2309031"

Revision as of 12:25, 1 November 2017

Standard forward primer for Gibson Assembly to plasmid pNZ8148

This primer contains 18 bp of the standard biobrick prefix, and a 20-bp-long overhang for Gibson Assembly, which can be digested and ligated into pNZ8148 at the restriction site PstI.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 22
Illegal PstI site found at 17
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 22
Illegal PstI site found at 17
Illegal NotI site found at 28
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 22
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 22
Illegal PstI site found at 17
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 22
Illegal PstI site found at 17
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2309031 short</partinfo>
-This primer contains 18 bp of the standardized biobrick prefix, and a 20-bp-long overhang for Gibson Assembly, which can be digested and ligated into pNZ8148 at the restriction site PstI.
+This primer contains 18 bp of the standard biobrick prefix, and a 20-bp-long overhang for Gibson Assembly, which can be digested and ligated into pNZ8148 at the restriction site PstI.
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