Difference between revisions of "Part:BBa K2398504:Design"
 
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<partinfo>BBa_K2398504 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2398504 SequenceAndFeatures</partinfo>
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This part is the coding sequence of the E. coli beta-lactamase. <br><br>
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This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3. <br><br>
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In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
 
In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
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===Design Notes===
 
===Design Notes===
 
compatible with RFC10
 
compatible with RFC10

Latest revision as of 15:48, 1 November 2017


lac_ Y103D_L167G_P164L


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This part is the coding sequence of the E. coli beta-lactamase.

In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).

Design Notes

compatible with RFC10



Source

genomic with modification

References