Difference between revisions of "Part:BBa K2448034"

Revision as of 11:03, 1 November 2017

D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens

This is the original sequence of the D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens str. C58 (Atu4750, UniProt A9CH28, BBa_K2448022) equipped with a strong RBS (BBa_K2448020).

Usage and Biology

D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-psicose being the carbon-3 (C3) epimer of D-fructose.

The A. tumefaciens Dpe is a 31.56 kDa protein of 289 amino acids.

This enzyme is highly specific for D-Psicose, but it is also able to catalyse with very low activity the epimerization of other D-ketoses (in the decreased substrate specificity) : D-fructose, D-tagatose, D-ribulose, D-xylulose and D-sorbose [1].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 208
Illegal NgoMIV site found at 268
Illegal NgoMIV site found at 619
Illegal AgeI site found at 646
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 174

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 __NOTOC__
 <partinfo>BBa_K2448034 short</partinfo>
-D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 This is the original sequence of the D-Psicose 3-epimerase (Dpe) from ''Agrobacterium tumefaciens'' str. C58 (Atu4750, UniProt A9CH28, [[Part:BBa_K2448022|BBa_K2448022]]) equipped with a strong RBS ([[Part:BBa_K2448020|BBa_K2448020]]).