Difference between revisions of "Part:BBa K2448050:Design"
 
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===References===
 
===References===
 +
 +
[1] de Boer HA, Comstock LJ, Vasser M. The tac promoter: A functional hybrid derived from the trp and lac promoters. Proc Natl Acad Sci U S A (1983) 80, 21-25.
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[2] [http://regprecise.lbl.gov/RegPrecise/sites.jsp?regulog_id=5087 RegPrecise database]

Latest revision as of 22:17, 1 November 2017


pPsiTac2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR (BBa_K2448006). A second consensus binding sequence of PsiR was added before the -35 box.

Source

DNA synthesis

References

[1] de Boer HA, Comstock LJ, Vasser M. The tac promoter: A functional hybrid derived from the trp and lac promoters. Proc Natl Acad Sci U S A (1983) 80, 21-25.

[2] [http://regprecise.lbl.gov/RegPrecise/sites.jsp?regulog_id=5087 RegPrecise database]