Difference between revisions of "Part:BBa K190031"
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The metallothionein (BBa_K190031) is a fMt(BBa_K190019) under control of a low constitutive promotor (BBa_J23109). We failed several times in replicating the ligation of these two parts. After sequencing BBa_K190031, BBa_K190019, and BBa_J23109, we found the constitutive promoter BBa_J23109 has two Spel restriction sites in the prefix.(Figure 2.) Thus, we decided to modify the biobrick by ligating fMt(BBa_K190019) with another constitutive promoter (BBa_J23119). Figure 3 shows the electrophoresis of BBa_K190019 when its plasmid was cut by XbaI and PstI. And Figure 4 shows the electrophoresis of BBa_J23119 and BBa_J23109 when their plasmids were cut by SpeI and PstI.
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<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2262015">BBa_K2262015</a></html></b></h3>
 
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Revision as of 10:26, 1 November 2017

fMT with low constitutive promoter

The metallothionein fMT under control of a low constitutive promotor BBa_J23109


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 170
  • 1000
    COMPATIBLE WITH RFC[1000]



(links to uploads relevant to your contribution)

Contribution

Group: NCTU_Formosa 2017

Author: Yi-Ting Lin, Huei-Yu Yeh, Sheng-Ping Chu

Summary:
The metallothionein (BBa_K190031) is a fMt(BBa_K190019) under control of a low constitutive promotor (BBa_J23109). We failed several times in replicating the ligation of these two parts. After sequencing BBa_K190031, BBa_K190019, and BBa_J23109, we found the constitutive promoter BBa_J23109 has two Spel restriction sites in the prefix.(Figure 2.) Thus, we decided to modify the biobrick by ligating fMt(BBa_K190019) with another constitutive promoter (BBa_J23119). Figure 3 shows the electrophoresis of BBa_K190019 when its plasmid was cut by XbaI and PstI. And Figure 4 shows the electrophoresis of BBa_J23119 and BBa_J23109 when their plasmids were cut by SpeI and PstI.

New improved part BBa_K2262015