Difference between revisions of "Part:BBa K2448050"

 
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This part is a hybrid synthetic promoter composed of the -35 and the Pribnow box sequences of pTacI promoter ([[Part:BBa_K864400|BBa_K864400]]) [1] and two consensus binding site of PsiR regulator of Rhizobiale [2].
 
This part is a hybrid synthetic promoter composed of the -35 and the Pribnow box sequences of pTacI promoter ([[Part:BBa_K864400|BBa_K864400]]) [1] and two consensus binding site of PsiR regulator of Rhizobiale [2].
  
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===Usage and Biology===
 
===Usage and Biology===
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D-Psicose inducible promoters naturally occur in nature, but they are quite long and not well characterized. In order to assess the efficiency of our psicose biosensor(s), we engineered a strong promoter, pTacI ([[Part:BBa_K864400|BBa_K864400]]), regulated by LacI to make it psicose inducible.
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pPsiTac2 is a synthetic promoter similar to [[Part:BBa_K2448016|BBa_K2448016]]. It is derived from pTacI ([[Part:BBa_K864400|BBa_K864400]]) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR interacting with psicose ([[Part:BBa_K2448006|BBa_K2448006]]). In pPsiTac2 (compared to [[Part:BBa_K2448016|BBa_K2448016]]) there is a second binding site for PsiR upstream the -35 box.
  
 
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Revision as of 10:36, 1 November 2017


pPsiTac2

This part is a hybrid synthetic promoter composed of the -35 and the Pribnow box sequences of pTacI promoter (BBa_K864400) [1] and two consensus binding site of PsiR regulator of Rhizobiale [2].

Usage and Biology

D-Psicose inducible promoters naturally occur in nature, but they are quite long and not well characterized. In order to assess the efficiency of our psicose biosensor(s), we engineered a strong promoter, pTacI (BBa_K864400), regulated by LacI to make it psicose inducible.

pPsiTac2 is a synthetic promoter similar to BBa_K2448016. It is derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR interacting with psicose (BBa_K2448006). In pPsiTac2 (compared to BBa_K2448016) there is a second binding site for PsiR upstream the -35 box.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]