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| − | For more Details visit our [http://2017.igem.org/Team:AQA_Unesp/Parts] | + | For more Details visit our [http://2017.igem.org/Team:AQA_Unesp/Parts webpage] |
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Revision as of 10:14, 1 November 2017
Applications of BBa_K305008
User Reviews
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victorNJ
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we (AQA_Unesp 2017) made a comparison between srfA and Pveg promoter, which is recognized
as being a strong one and is already featured in the registry (BBa_K143012),
and we show a relationship of cell growth and expression of GFP.
As a tool for measuring the activity of promoters, reporter vectors can be required. Therefore, we chose the luciferase
reporter (pBs3Clux-PsrfA), and green fluorescence protein reporter (pBs1C-PsrfA-GFP). For luciferase reporter, we did the
percentage of luminescence making the ratio by the point of greatest luminescence and multiplying by 100.
Already by GFP, we measured at 535nm for emission and 285nm for excitation.
The readings were performed on the Infinite® M200 (Tecan).
For more Details visit our [http://2017.igem.org/Team:AQA_Unesp/Parts webpage]
Evaluation
 Figure 1. . Growth curve of Bacillus subitilis for about 7 hour, 37°C and 220 rpm.  Figure 2. . Percentage of Luminescence of PsrfA and Pveg; We use for Positive control the plasmid containing pBs3Clux without promoter. The growth has occurred for about 7 hour, 37°C and 220 rpm.  Figure 3. . Correlation of GFP expression (pBs1C-srfA-GFP) and cell growth as a function of time for about 8 hour, 37°C and 220 rpm.
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