Difference between revisions of "Part:BBa K305008:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
 
===Applications of BBa_K305008===
 
===Applications of BBa_K305008===
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===User Reviews===
 
===User Reviews===
 
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Revision as of 09:52, 1 November 2017


Applications of BBa_K305008

User Reviews

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victorNJ 

we (AQA_Unesp 2017) made a comparison between srfA and Pveg promoter, which is recognized as being a strong one and is already featured in the registry (BBa_K143012), and we show a relationship of cell growth and expression of GFP. As a tool for measuring the activity of promoters, reporter vectors can be required. Therefore, we chose the luciferase reporter (pBs3Clux-PsrfA), and green fluorescence protein reporter (pBs1C-PsrfA-GFP). For luciferase reporter, we did the percentage of luminescence making the ratio by the point of greatest luminescence and multiplying by 100. Already by GFP, we measured at 535nm for emission and 285nm for excitation. The readings were performed on the Infinite® M200 (Tecan)


For more Details visit our [http://2017.igem.org/Team:AQA_Unesp/Parts]

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