Difference between revisions of "Part:BBa K2457006"
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===Usage and Biology===
 
===Usage and Biology===
  
 +
===Design===
 +
 +
[[File:BBa K2457006 circuit.png]]
 +
 +
Figure 1: BBa K2457006 circuit
  
 
===Characterization===
 
===Characterization===
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[[File:BBa K2457006 electropherogram.png]]
 
[[File:BBa K2457006 electropherogram.png]]
  
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Figure 1:Sequencing electropherogram from BBa_K2457006.  
+
Figure 2:Sequencing electropherogram from BBa_K2457006.  
  
 
[[File:BBa K2457006 alignment.png]]
 
[[File:BBa K2457006 alignment.png]]
  
−
Figure 2:Alignment of the designed sequence and our final construction from BBa_K2457006.
+
Figure 3:Alignment of the designed sequence and our final construction from BBa_K2457006.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 09:53, 1 November 2017


Cas9 device + Optimized sgRNA

descrever

Usage and Biology

Design

BBa K2457006 circuit.png

Figure 1: BBa K2457006 circuit

Characterization

BBa K2457006 electropherogram.png

Figure 2:Sequencing electropherogram from BBa_K2457006.

BBa K2457006 alignment.png

Figure 3:Alignment of the designed sequence and our final construction from BBa_K2457006.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1024