Difference between revisions of "Part:BBa K1875003:Experience"
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==Characterization by ''' [http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] ''' == | ==Characterization by ''' [http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] ''' == | ||
| − | We cultured <i>B. xylophilus</i> with <i>S. cerevisiae</i> expressing EGFP of this part from the TDH3 promoter (BBa_K530008). After several days we collected the nematodes and observed them with a fluorescence microscope, and we were able to confirm nematodes emitting green fluorescence throughout the intestinal tract. | + | We cultured <i>B. xylophilus</i> with <i>S. cerevisiae</i> expressing EGFP of this part from the TDH3 promoter (''' [https://parts.igem.org/Part:BBa_K530008 BBa_K530008] ''' ). After several days we collected the nematodes and observed them with a fluorescence microscope (fig 1 ), and we were able to confirm nematodes emitting green fluorescence throughout the intestinal tract. |
| − | + | <html> | |
| − | < | + | <figure> |
| + | <img src="https://static.igem.org/mediawiki/parts/8/82/B._xEGFP.jpg" alt="<i>B. xylophilus</i> filled with EGFP in its intestine" style="float:center;width:350px;height:250px;"> | ||
| + | </figure> | ||
| + | Figure1: <i>B. xylophilus</i> full of EGFP in its esophagus. | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 11:06, 1 November 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1875003
Characterization by [http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017]
We cultured B. xylophilus with S. cerevisiae expressing EGFP of this part from the TDH3 promoter ( BBa_K530008 ). After several days we collected the nematodes and observed them with a fluorescence microscope (fig 1 ), and we were able to confirm nematodes emitting green fluorescence throughout the intestinal tract.
User Reviews
UNIQ943d4f4a7f4a6001-partinfo-00000001-QINU UNIQ943d4f4a7f4a6001-partinfo-00000002-QINU