Difference between revisions of "Part:BBa K2446035:Design"
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===Source=== | ===Source=== | ||
| − | + | this part is assembled by Sv40 promoter BBa_K2446052 and 2*PIP DNA binding sites BBa_K2446017. | |
===References=== | ===References=== | ||
Revision as of 03:41, 1 November 2017
SV40_8_PIP promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is aseembled by the specfic DNA binding sites and Sv40 promoter through Biobrick assembly. We inset the DNA binding sites behind the Sv40 promoter. Then the DNA binding domain with KRAB can repress the expression of this promoter. The repression intensity can be adjusted by change the repeats of DNA binding sites.
Source
this part is assembled by Sv40 promoter BBa_K2446052 and 2*PIP DNA binding sites BBa_K2446017.