Difference between revisions of "Part:BBa K2213012"
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<partinfo>BBa_K2213012 short</partinfo>
 
<partinfo>BBa_K2213012 short</partinfo>
  
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===Characterisation===
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This part was expressed alongside EutLK-Low-PduD-mCherry-PPK (https://parts.igem.org/Part:BBa_K2213013) to form EutSMNLK+Low_PduD+PPK which was characterized as follows.<br>
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<br>
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A 24 hour induction was DAPI stained to determine microcompartment formation and tag localisation.<br>
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<br>
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https://static.igem.org/mediawiki/2017/4/4f/LowSMNLKDAPIcells.png<br>
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<b>Figure 1.</b> Fluorescence microscopy images of promoter-PduD associated mCherry and DAPI stained polyphosphate.<br>
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<br>
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As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits.
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<br>
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<partinfo>BBa_K2213012 parameters</partinfo>
 
<partinfo>BBa_K2213012 parameters</partinfo>
 
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Function:<br>
 
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This part was expressed alongside EutLK-Low-PduD-mCherry-PPK (https://parts.igem.org/Part:BBa_K2213013) to form EutSMNLK+Low_PduD+PPK which was characterized as follows.<br>
 
−
<br>
 
−
A 24 hour induction was DAPI stained to determine microcompartment formation and tag localisation.<br>
 
−
<br>
 
−
https://static.igem.org/mediawiki/2017/4/4f/LowSMNLKDAPIcells.png<br>
 
−
<b>Figure 2.</b> Fluorescence microscopy images of promoter-PduD associated mCherry and DAPI stained polyphosphate.<br>
 
−
<br>
 
−
As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits.
 
−
<br>
 

Revision as of 02:23, 1 November 2017


LacUV5_EutS_Tet_EutMN

Characterisation

This part was expressed alongside EutLK-Low-PduD-mCherry-PPK (https://parts.igem.org/Part:BBa_K2213013) to form EutSMNLK+Low_PduD+PPK which was characterized as follows.

A 24 hour induction was DAPI stained to determine microcompartment formation and tag localisation.

LowSMNLKDAPIcells.png
Figure 1. Fluorescence microscopy images of promoter-PduD associated mCherry and DAPI stained polyphosphate.

As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3451