Difference between revisions of "Part:BBa K2213002"
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<b> <font size="+0.7"> Fig 1: Schematic of the araBAD_EutLK part (BBa_K2213002) </font></b> | <b> <font size="+0.7"> Fig 1: Schematic of the araBAD_EutLK part (BBa_K2213002) </font></b> | ||
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<u><font size="+0.5">araBad Promoter</font></u> | <u><font size="+0.5">araBad Promoter</font></u> | ||
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The ara expression system is advantageous as in addition to being induced by arabinose, gene expression can be rapidly downregulated as repression can occur due to catabolite repression in the presence of glucose or by competitive binding of the anti-inducer fucose. Plasmids containing the ara expression system have minimal levels of background expression (Siegele and Hu, 1997). | The ara expression system is advantageous as in addition to being induced by arabinose, gene expression can be rapidly downregulated as repression can occur due to catabolite repression in the presence of glucose or by competitive binding of the anti-inducer fucose. Plasmids containing the ara expression system have minimal levels of background expression (Siegele and Hu, 1997). | ||
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<u><font size="+0.5">EutLK</font></u> | <u><font size="+0.5">EutLK</font></u> | ||
Revision as of 01:40, 1 November 2017
araB_eutLK
The ethanolamine utilisation bacterial microcompartment (BMC) proteins, EutLK (from E. coli) under control of the arabinose inducible promoter. Contains RBS, terminators and all tetp components. EutL is tagged with His6. EutK is tagged with FLAG (see figure 1).
Fig 1: Schematic of the araBAD_EutLK part (BBa_K2213002)
araBad Promoter
The ara expression system is advantageous as in addition to being induced by arabinose, gene expression can be rapidly downregulated as repression can occur due to catabolite repression in the presence of glucose or by competitive binding of the anti-inducer fucose. Plasmids containing the ara expression system have minimal levels of background expression (Siegele and Hu, 1997).
EutLK
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1300
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1239
Illegal BamHI site found at 2164 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1074
Illegal AgeI site found at 1775
Illegal AgeI site found at 1952
Illegal AgeI site found at 2252
Illegal AgeI site found at 2315
Illegal AgeI site found at 2362 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1056
References
Siegele, D.A. and Hu, J.C., 1997. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proceedings of the National Academy of Sciences, 94(15), pp.8168-8172.