Difference between revisions of "Part:BBa K2443019"

Latest revision as of 18:47, 1 November 2017

TrpRS optimized for expression in E. coli

Tryptophan tRNA synthetase responsible for attaching Tryptophan onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of TrpRS is 39 kDa, which corresponds to the band seen in lane 5 of the overexpression gel (below). Overexpressions were performed according to the protein overexpression protocol and lab notebook on the 2017 iGEM lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 682
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2443019 short</partinfo>
-Tryptophan tRNA synthetase responsible for attaching Tryptophan onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.
+Tryptophan tRNA synthetase responsible for attaching Tryptophan onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of TrpRS is 39 kDa, which corresponds to the band seen in lane 5 of the overexpression gel (below). Overexpressions were performed according to the protein overexpression protocol and lab notebook on the 2017 iGEM lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf)
+https://static.igem.org/mediawiki/parts/b/bf/Lethbridge_Oct_22_gel.png