Difference between revisions of "Part:BBa K2377006"

Revision as of 22:36, 31 October 2017

TrxzN, a developed element to generate trxz hpRNA with a intron loop

As the intron sequence will be sliced during the pre-mRNA processing and the stability is much higher than dsRNA and hpRNA with a normal loop according to a relevant paper, it will be a better option for us to silence the target sequene. So we constructed this composite part to generate Ecr hpRNA with a intron loop.

The ecdysone receptor (ECR) is a such kind of gene, which could up-regulate the vital genes expression through the interaction with ultra-stable protein (USP) under the existence of ecdysteroid hormone. Therefore, Ecr gene silencing can effectively increasing the insects mortality and reducing the reproduction rate. Studies have shown when fed aphids with 7.5 ng / ul dsRNA Ecr, the mortality rate up to 70% after 8 days. According to the literature, the similarity of Ecr gene sequence between pea aphid and grain aphid was 98.4% and with more than 90% similarity in other kind of aphids, so Ecr is an Ideal RNAi target site to kill aphids with a broad spectrum destruction effect.

Sequence and Features