Difference between revisions of "Part:BBa K2457001"
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[[File:BBa K2457001 electropherogram.png]] | [[File:BBa K2457001 electropherogram.png]] | ||
| − | + | Figure 1:Sequencing electropherogram from BBa_K2457000. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2457001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2457001 SequenceAndFeatures</partinfo> | ||
Revision as of 21:41, 31 October 2017
Standardized Cas9 coding sequence + B0015 terminator
This brick was engineered to be a building block of the standard BioBrick vector for one-step bacterial genome engineering: the ([http://2017.igem.org/Team:Amazonas_Brazil pCRISPeasy]). BBa_K2457001 is composed of the Cas9 coding sequence from Streptococcus pyogenes + BBa_B0015 double terminator. It was rationally designed to be interchangeable AND standardized, providing a BioBrick to attend multiple SynBio purposes. For that matter, the RBS and promoter are easily switchable through 3A Assembly. It’s also standard restriction enzymes safe for all RFCs assemblies.
Usage and Biology
Design
Characterization
Figure 1:Sequencing electropherogram from BBa_K2457000.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]