Difference between revisions of "Part:BBa K2457000"
(→‎Usage and Biology)
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[[File:AraC pBAD alignment.png]]
 
[[File:AraC pBAD alignment.png]]
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Figure 1:Allignment of the designed sequence and our final construction from BBa_K2457000.  
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Figure 2:Allignment of the designed sequence and our final construction from BBa_K2457000.  
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 21:15, 31 October 2017


araC + pBAD arabinose inducible promoter

Arabinose inducible promoter pBAD forward + araC regulatory reverse gene under control of pC promoter and tryptophan terminator sequence.


Usage and Biology

AraC pBAD electropherogram sequence1.jpeg Figure 1:Sequencing electropherogram from BBa_K2457000.

AraC pBAD alignment.png Figure 2:Allignment of the designed sequence and our final construction from BBa_K2457000.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1024