Difference between revisions of "Part:BBa K2463002:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
https://parts.igem.org/Part:BBa_K2463000
+
Lacks a degrading tag fused within yhjH gene due to unpredictable interactions.
  
  
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===Source===
 
===Source===
  
−
https://parts.igem.org/Part:BBa_K2463000
+
====Uniprot entries for important proteins:====
 +
PLeD Source: [http://www.uniprot.org/uniprot/Q9A5I5 uniprot.org/uniprot/Q9A5I5]
 +
 
 +
yhjH Source: [http://www.uniprot.org/uniprot/P37646 uniprot.org/uniprot/P37646]
 +
 
 +
===iGEM Registry Parts:===
 +
[https://parts.igem.org/Part:BBa_R0082 BBa_R0082] - Promoter (OmpR, positive)
 +
 
 +
[https://parts.igem.org/Part:BBa_K2463000 BBa_K2463000] - Biofilm and Motility Control Device
 +
 
 +
====[https://parts.igem.org/Part:BBa_K2463000 Biofilm and Motility Control Device Affiliated Parts]:====
 +
[https://parts.igem.org/Part:BBa_B0034 BBa_B0034] - Ribosome binding site
 +
 
 +
[https://parts.igem.org/Part:BBa_K2012002 BBa_K2012002] - <b>PLeD gene</b> <i>(synthesises c-di-GMP)</i>
 +
 
 +
[https://parts.igem.org/Part:BBa_C0072 BBa_C0072] - Mnt repressor
 +
 
 +
[https://parts.igem.org/Part:BBa_B1006 BBa_B1006] - Terminator
 +
 
 +
[https://parts.igem.org/Part:BBa_R0073 BBa_R0073] - Mnt promoter
 +
 
 +
[https://parts.igem.org/Part:BBa_K861090 BBa_K861090] - <b>Yhjh</b> <i>(degrades c-di-GMP)</i>
 +
 
 +
[https://parts.igem.org/Part:BBa_B1003 BBa_B1003] - Second Terminator
  
 
===References===
 
===References===

Latest revision as of 20:14, 31 October 2017


OmpR Controlled Biofilm and Motility Regulation Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1922
    Illegal BamHI site found at 148
    Illegal BamHI site found at 1306
    Illegal XhoI site found at 1884
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 564
    Illegal NgoMIV site found at 711
    Illegal AgeI site found at 1047
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 221


Design Notes

Lacks a degrading tag fused within yhjH gene due to unpredictable interactions.


Source

Uniprot entries for important proteins:

PLeD Source: [http://www.uniprot.org/uniprot/Q9A5I5 uniprot.org/uniprot/Q9A5I5]

yhjH Source: [http://www.uniprot.org/uniprot/P37646 uniprot.org/uniprot/P37646]

iGEM Registry Parts:

BBa_R0082 - Promoter (OmpR, positive)

BBa_K2463000 - Biofilm and Motility Control Device

Biofilm and Motility Control Device Affiliated Parts:

BBa_B0034 - Ribosome binding site

BBa_K2012002 - PLeD gene (synthesises c-di-GMP)

BBa_C0072 - Mnt repressor

BBa_B1006 - Terminator

BBa_R0073 - Mnt promoter

BBa_K861090 - Yhjh (degrades c-di-GMP)

BBa_B1003 - Second Terminator

References