Difference between revisions of "Part:BBa J23105"
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<partinfo>BBa_J23105 parameters</partinfo>
 
<partinfo>BBa_J23105 parameters</partinfo>
 
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<strong>Manchester 2017</strong> used this part to create part LowPromoter_PduD(1-20)_mCherry (BBa_K2213006). This promoter was combined with PduD(1-20) to create a tag with lower expression levels. The mCherry tagged PduD(1-20) localisation tag displayed lower fluorescence levels under the low promoter as compared to under medium (BBa_K2213007) and high strength (BBa_K2213008) promoters, demonstrating correct function.<br>
 
<strong>Manchester 2017</strong> used this part to create part LowPromoter_PduD(1-20)_mCherry (BBa_K2213006). This promoter was combined with PduD(1-20) to create a tag with lower expression levels. The mCherry tagged PduD(1-20) localisation tag displayed lower fluorescence levels under the low promoter as compared to under medium (BBa_K2213007) and high strength (BBa_K2213008) promoters, demonstrating correct function.<br>

Revision as of 19:56, 31 October 2017

constitutive promoter family member

BerkiGEM2006-PromotersEppendorfs.jpg
BerkiGEM2006-Promoters.jpg

 
 Variant RFP (au)
 J23112           1
 J23103           17
 J23113           21
 J23109           106
 J23117           162
 J23114           256
 J23115           387
 J23116           396
 J23105           623
 J23110           844
 J23107           908
 J23106           1185
 J23108           1303
 J23118           1429
 J23111           1487
 J23101           1791
 J23104           1831
 J23102           2179
 J23100           2547
PBca1020-r0040.jpg

Constitutive promoter family
Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. J23119 is the "consensus" promoter sequence and the strongest member of the family. All parts except J23119 are present in plasmid J61002. Part J23119 is present in pSB1A2. This places the RFP downstream of the promoter. Reported activities of the promoters are given as the relative fluorescence of these plasmids in strain TG1 grown in LB media to saturation. See part BBa_J61002 for details on their use.

These promoter parts can be used to tune the expression level of constitutively expressed parts. The NheI and AvrII restriction sites present within these promoter parts make them a scaffold for further modification. JCAraw




Manchester 2017 used this part to create part LowPromoter_PduD(1-20)_mCherry (BBa_K2213006). This promoter was combined with PduD(1-20) to create a tag with lower expression levels. The mCherry tagged PduD(1-20) localisation tag displayed lower fluorescence levels under the low promoter as compared to under medium (BBa_K2213007) and high strength (BBa_K2213008) promoters, demonstrating correct function.

More information can be found here: https://parts.igem.org/Part:BBa_K2213006

TagExpression500p.jpg