Difference between revisions of "Part:BBa K2235006"

Revision as of 19:36, 31 October 2017

Sialidase enzyme with a RBS attached

This part constitutes of RBS functional unit attached to sialidase basic part (BBa_K2235005) that can be used to test on various promoters. BBa_K2235007 biobrick has the characterisation for an OmpR promoter fused to this (BBa_K2235006) part. The following parts contain the sialidase enzyme:
Basic part BBa_K2235005 consists of the sialidase enzyme coding sequence with a His tag C-terminally attached.
BBa_K2235009 biobrick is a composite of a T7 promoter and RBS followed by sialidase with characterisation data of the enzyme.
BBa_K2235011 is a conjugation of sialidase composite (BBa_K2235009) with a device that facilitates the secretion of the enzyme out of the E.coli bacterial cell.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 73
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 520
Illegal NgoMIV site found at 595
Illegal NgoMIV site found at 685
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1065

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2235006 short</partinfo>
 <p>
-This part constitutes of RBS functional unit attached to sialidase basic part (BBa_K2235005) that can be used to test on various promoters. BBa_K2235007 biobrick has the characterisation for an OmpR promoter fused to this (BBa_K2235006) part. Further, the following parts contain the sialidase enzyme:
+This part constitutes of RBS functional unit attached to sialidase basic part (BBa_K2235005) that can be used to test on various promoters. BBa_K2235007 biobrick has the characterisation for an OmpR promoter fused to this (BBa_K2235006) part. The following parts contain the sialidase enzyme:
 <br>Basic part BBa_K2235005 consists of the sialidase enzyme coding sequence with a His tag C-terminally attached.