Difference between revisions of "Part:BBa K895006"
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− | Summary: iGEM 2017 Team Tsinghua has made a significant improvement of this part, by validating that this GFP protein works well in the S. cerevisiae (baking yeast) chassis, and adding | + | Summary: iGEM 2017 Team Tsinghua has made a significant improvement of this part, by validating that this GFP protein works well in the S. cerevisiae (baking yeast) chassis, and adding a yeast inducible promoter pGalS (BBa_K2247000) in front of this part. The new part we constructed is [https://parts.igem.org/Part:BBa_K2247001 BBa_K2247001]. By making this improvement, we achieved inducible expression of GFP by galactose or by yeast Gal4 protein in yeast cells, and this new composite part can also serve to validate and characterize the function of the pGalS promoter. In future projects applying the yeast-two-hybrid or yeast-one-hybrid system, this new part can serve as a basic and standard reporter plasmid. For experimental validation of the new part, please see the main page of BBa_K2247001. |
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Revision as of 19:59, 31 October 2017
GFP open reading frame
This is GFP from Biobrick BBa_J54103, which we have sequenced and re-cloned to RFC[10] standard in pSB1C3.
Contribution
Group: Team Tsinghua 2017
Author: Boyang Gao
Summary: iGEM 2017 Team Tsinghua has made a significant improvement of this part, by validating that this GFP protein works well in the S. cerevisiae (baking yeast) chassis, and adding a yeast inducible promoter pGalS (BBa_K2247000) in front of this part. The new part we constructed is BBa_K2247001. By making this improvement, we achieved inducible expression of GFP by galactose or by yeast Gal4 protein in yeast cells, and this new composite part can also serve to validate and characterize the function of the pGalS promoter. In future projects applying the yeast-two-hybrid or yeast-one-hybrid system, this new part can serve as a basic and standard reporter plasmid. For experimental validation of the new part, please see the main page of BBa_K2247001.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644