Difference between revisions of "Part:BBa K530008"
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==<big>''' CONTRIBUTION '''</big>==
 
==<big>''' CONTRIBUTION '''</big>==
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''' [http://2017.igem.org/Team:Kyoto  iGEM Kyoto 2017] ''' <big>improved</big> this part by connecting this part with ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003(EGFP)] ''' and added further functions. In this improvement, we obtained a new part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] '''.
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''' [http://2017.igem.org/Team:Kyoto  iGEM Kyoto 2017] ''' <big>improved</big> this part by connecting it with ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003(EGFP)] ''', adding further functions. In this improvement, we obtained a new part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] '''.
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===Combination with BBa_K1875003(EGFP)===
 
===Combination with BBa_K1875003(EGFP)===
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With this combination, this part ''' [https://parts.igem.org/Part:BBa_K530008 BBa_K530008 (TDH3 promoter)] ''' was used to create the part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] ''' which codes EGFP emitting strong green fluorescence. As a result, it was possible to confirm yeast emitting a strong green fluorescence under the microscope (photo or link). In addition, we confirmed that the pine wood nematode eats <i>S. cerevisiae</i>. Such usage is innovative and has greatly contributed to our project.
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We combined this part (BBa_K530008, TDH 3 promoter) with BBa_K1875003 (EGFP expression vector for yeast. We validated the plasmid in <i>S. cerevisiae,</i> and detected a strong green fluorescence under the microscope (photo or link). In addition, we confirmed that pine wood nematodes eat <i>S. cerevisiae</i>. This innovation greatly contributed to our project.

Revision as of 17:19, 31 October 2017

TDH3 Yeast Promoter

TDH3 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Map

TDH3p Plasmid Map.png


Sequencing

TDH3p Sequencing.png


This is the sequencing for colony 10. Both colony 1 and 10 were a perfect match with the genomic DNA promoter.


CONTRIBUTION

[http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] improved this part by connecting it with BBa_K1875003(EGFP) , adding further functions. In this improvement, we obtained a new part BBa_K2403003 .


Combination with BBa_K1875003(EGFP)

We combined this part (BBa_K530008, TDH 3 promoter) with BBa_K1875003 (EGFP expression vector for yeast. We validated the plasmid in S. cerevisiae, and detected a strong green fluorescence under the microscope (photo or link). In addition, we confirmed that pine wood nematodes eat S. cerevisiae. This innovation greatly contributed to our project.