Difference between revisions of "Part:BBa K530008"
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''' [http://2017.igem.org/Team:Kyoto  iGEM Kyoto 2017] '''  <big>improved</big> this part by connecting this part with ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003(EGFP)] ''' and added further functions. In this improvement, we obtained a new part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] '''.
 
''' [http://2017.igem.org/Team:Kyoto  iGEM Kyoto 2017] '''  <big>improved</big> this part by connecting this part with ''' [https://parts.igem.org/Part:BBa_K1875003  BBa_K1875003(EGFP)] ''' and added further functions. In this improvement, we obtained a new part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] '''.
  
−
===Combination with BBa_K530008 (TDH3 promoter)===
+
===Combination with BBa_K1875003(EGFP)===
 
With this combination, this part ''' [https://parts.igem.org/Part:BBa_K530008  BBa_K530008 (TDH3 promoter)] ''' was used to create the part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] ''' which codes EGFP emitting strong green fluorescence. As a result, it was possible to confirm yeast emitting a strong green fluorescence under the microscope (photo or link). In addition, we confirmed that the pine wood nematode eats <i>S. cerevisiae</i>. Such usage is innovative and has greatly contributed to our project.
 
With this combination, this part ''' [https://parts.igem.org/Part:BBa_K530008  BBa_K530008 (TDH3 promoter)] ''' was used to create the part ''' [https://parts.igem.org/Part:BBa_K2403003  BBa_K2403003] ''' which codes EGFP emitting strong green fluorescence. As a result, it was possible to confirm yeast emitting a strong green fluorescence under the microscope (photo or link). In addition, we confirmed that the pine wood nematode eats <i>S. cerevisiae</i>. Such usage is innovative and has greatly contributed to our project.

Revision as of 16:27, 31 October 2017

TDH3 Yeast Promoter

TDH3 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Map

TDH3p Plasmid Map.png


Sequencing

TDH3p Sequencing.png


This is the sequencing for colony 10. Both colony 1 and 10 were a perfect match with the genomic DNA promoter.


CONTRIBUTION

[http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] improved this part by connecting this part with BBa_K1875003(EGFP) and added further functions. In this improvement, we obtained a new part BBa_K2403003 .

Combination with BBa_K1875003(EGFP)

With this combination, this part BBa_K530008 (TDH3 promoter) was used to create the part BBa_K2403003 which codes EGFP emitting strong green fluorescence. As a result, it was possible to confirm yeast emitting a strong green fluorescence under the microscope (photo or link). In addition, we confirmed that the pine wood nematode eats S. cerevisiae. Such usage is innovative and has greatly contributed to our project.