Difference between revisions of "Part:BBa I751501"
Line 4: Line 4:
 
sense TWO INPUTS, activation by AHL and repression by Lambda cI.
 
sense TWO INPUTS, activation by AHL and repression by Lambda cI.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
[[Image:Hybridgraph.JPG|thumb|300px| '''Fig.3: The result of assay0''' <br>Only in the presence of AHL and in the absence of lacI, the lux lac hybrid promoter is activated significantly.|right]]
 +
The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though '''[[http://parts.mit.edu/igem07/index.php/Tokyo/Works/Assay0 |Assay 0]]'''. The results are shown in Fig.3.
 +
'''The newly devised hybrid promoter is activated by AHL and repressed by LacI.'''
 +
<br>In the present project, this hybrid promoter has been tested on the last year's A-4 BioBrick part (BBa_J54140) by replacing with its original promoter lux pR (R0062).
 +
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 15:54, 26 October 2007

plux-cI hybrid promoter

sense TWO INPUTS, activation by AHL and repression by Lambda cI.

Usage and Biology

Fig.3: The result of assay0
Only in the presence of AHL and in the absence of lacI, the lux lac hybrid promoter is activated significantly.

The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though Assay 0. The results are shown in Fig.3. The newly devised hybrid promoter is activated by AHL and repressed by LacI.
In the present project, this hybrid promoter has been tested on the last year's A-4 BioBrick part (BBa_J54140) by replacing with its original promoter lux pR (R0062).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]