Difference between revisions of "Part:BBa K2377008"

Revision as of 22:53, 31 October 2017

EcrN, a developed element to generate Ecr hpRNA with intron loop

We designed this composite biobrick for producing Ecr hpRNA with intron. Acyrthosiphon pisum ecdysone receptor (Ecr) is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003383721.1). (ECR) could up-regulate the aphids vital genes expression through the interaction with ultra-stable protein (USP) under the existence of ecdysteroid hormone. Therefore, Ecr gene silencing can effectively increase the insects mortality and reduce reproduction rate. Studies have shown when fed aphids with 7.5 ng / ul dsRNA Ecr, the mortality rate up to 70% after 8 days. According to the literature, the similarity of Ecr gene sequence between pea aphid and grain aphid was 98.4% and with more than 90% similarity in other kind of aphids, so Ecr is an Ideal RNAi target site to kill aphids with a broad spectrum destruction effect. one element to genarete hpRNA. ECR silencing can effectively reduce the expression of these key genes, thereby reducing the survival rate of insects and reproduction rate. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 368
Illegal BglII site found at 968
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 929
Illegal BsaI.rc site found at 407

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 <partinfo>BBa_K2377008 short</partinfo>
-An Aphid intron between sense and antisense of Ecr fragment generate hpRNA targeting Ecr gene in Aphid.
+We designed this composite biobrick for producing Ecr hpRNA with intron.
+Acyrthosiphon pisum ecdysone receptor (Ecr) is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003383721.1). (ECR) could up-regulate the aphids vital genes expression through the interaction with ultra-stable protein (USP) under the existence of ecdysteroid hormone.
+Therefore, Ecr gene silencing can effectively increase the insects mortality and reduce reproduction rate. Studies have shown when fed aphids with 7.5 ng / ul dsRNA Ecr, the mortality rate up to 70% after 8 days.
+According to the literature, the similarity of Ecr gene sequence between pea aphid and grain aphid was 98.4% and with more than 90% similarity in other kind of aphids, so Ecr is an Ideal RNAi target site to kill aphids with a broad spectrum destruction effect. one element to genarete hpRNA.
+ECR silencing can effectively reduce the expression of these key genes, thereby reducing the survival rate of insects and reproduction rate.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===