Difference between revisions of "Part:BBa K2239002:Design"
Line 11: Line 11:
  
 
===Source===
 
===Source===
 +
 
Both the 7beta-HSDH and ChBD sequence are retrieved from the GenBank and artificially synthesized.
 
Both the 7beta-HSDH and ChBD sequence are retrieved from the GenBank and artificially synthesized.
  

Revision as of 14:52, 31 October 2017


ChBD-7betaHSDH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1131
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 965


Design Notes

The ChBD tag is added to purify the effective enzymes. When connected with the enzyme DNA and expressed, ChBD is able to bind to the chitin powder. As a result, the enzyme is successfully immobilized on the chitin powder inside the solution.


Source

Both the 7beta-HSDH and ChBD sequence are retrieved from the GenBank and artificially synthesized.


References

[1] Ming-Min Zheng, Ru-Feng Wang, Chun-Xiu Li, Jian-He Xu: Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Process Biochemistry, Elsevier, 2015.

[2] Takahisa Ikegami, Terumasa Okada, Masayuki Hashimoto, Shizuka Seino, Takeshi Watanabe, and Masahiro Shirakawa: Solution Structure of the Chitin-binding Domain of Bacillus circulans WL-12 Chitinase A1. The Journal Of Biological Chemistry, 2000.