Difference between revisions of "Part:BBa K2371012"

Revision as of 15:37, 31 October 2017

pT7-amilGFP

In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest.

Figure1.PCR test of eforRed and amliGFP

Figure2.RD test of eforRed and amlGFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 600
Illegal AgeI site found at 712
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2371012 short</partinfo>
-This chromoprotein eforRed naturally exhibits red/pink color when expressed. The color is slightly weaker than RFP. On agar plates and in liquid culture, the color is readily visible to naked eye in less than 24 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
-<h5>Figure1.PCR test of eforRed and GFP.</h5>
+In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest.
+<h5>Figure1.PCR test of eforRed and amliGFP</h5>
 [[File:Pcr test of eforRed and GFP.png|400px]]
-<h5>Figure2.RD test of eforRed and GFP.</h5>
+<h5>Figure2.RD test of eforRed and amlGFP.</h5>
 [[File:RD test of amliRed and GFP.png|400px]]