Difference between revisions of "Part:BBa K2273001:Design"
Line 7: Line 7:
  
 
===Design===
 
===Design===
−
This part was generated with RFC10 Suffix and Prefix. It encodes for the <i>srfA</i> promoter that is part of the ComX quorum response of <i>B. subtilis</i> and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].<br>
+
This part was generated with RFC10 Suffix and Prefix. It encodes for the <i>rapA</i> promoter that is part of the ComX quorum response of <i>B. subtilis</i> and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].<br>
  
 
===Source===
 
===Source===
−
The <i>srfA</i> promoter was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below.
+
The <i>rapA</i> promoter was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below.
 
<br>
 
<br>
 
<table>
 
<table>
 
<tr>
 
<tr>
−
<td width="150">PsrfA fwd RFC10</td>
+
<td width="150">PrapA fwd RFC10</td>
−
<td>gatcGAATTCGCGGCCGCTTCTAGAGTTGCGGCATCCCGCAAAAAATATTG</td>
+
<td>gatcGAATTCGCGGCCGCTTCTAGAGTTTGTTGCGGTTAGCCGAAATTC</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
<td>PsrfA rev RFC10</td>
+
<td>PrapA rev RFC10</td>
−
<td>gatcCTGCAGCGGCCGCTACTAGTACTTTATAAGCAGTGAACATG</td>
+
<td>gatcCTGCAGCGGCCGCTACTAGTATTTGAATTACCCGAGATATGTC</td>
 
</tr>
 
</tr>
 
</table>
 
</table>

Revision as of 11:19, 31 October 2017


PrapA: a ComA-dependent promoter of Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

This part was generated with RFC10 Suffix and Prefix. It encodes for the rapA promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].

Source

The rapA promoter was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

PrapA fwd RFC10 gatcGAATTCGCGGCCGCTTCTAGAGTTTGTTGCGGTTAGCCGAAATTC
PrapA rev RFC10 gatcCTGCAGCGGCCGCTACTAGTATTTGAATTACCCGAGATATGTC

References

Willenbacher J, Mohr T, Henkel M, Gebhard S, Mascher T, Syldatk C, Hausmann R (2016) Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production. Journal of Molecular Biology 362: 393-402. PubMed

Nakano MM, Xia LA, Zuber P (1991) Transcription initiation region of the srfA operon, which is controlled by the comP-comA signal transduction system in Bacillus subtilis. J Bacteriol. 173(17):5487-93. PubMed

Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB(2016) The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. Nucleic Acids Res.44(5):2160-72. PubMed

Hamoen LW, Venema G, Kuipers OP (2003) Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 149(Pt 1):9-17 PubMed