Difference between revisions of "Help:Submission Kit"

Revision as of 14:08, 4 September 2018

DNA Submission

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Sample submission of parts is no longer a requirement for the iGEM competition.

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Requirements

Here's how part samples are sent to the Registry

Why pSB1C3?

Why do samples need to be shipped in pSB1C3?

Detailed Instructions

Instructions on how to fill out the Registry's submission form.

Submission Prep

Registry groups must follow iGEM's submission prep guidelines.

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Have questions on requirements or shipping part samples? Check the FAQ or contact us at hq (at) igem . org .

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Submission Format

As in previous years, parts must be BioBrick compatible and in pSB1C3.

Additionally, all part submissions must be:

• miniprepped plasmid DNA (over 250ng) dried down. 10ul of 25ng/ul for a total of 250ng works well.
• in 96 well plate format (label your plate with your team name and submission number)
• plate sealed with adhesive foil
  • the adhesive foil should make full contact with the wells of the plate (see picture)
• sealed plate covered with a plastic lid
• shipped with a tracking number
• shipped and labelled as DNA, not hidden or disguised
  • DNA (non-hazardous, non-regulated, non-infectious, for research purposes only)

We've provided a submission kit with all of the materials you'll need to ship your parts. Read instructions below.

Submission Kit Contents

NOTE: The Submission Kit is new to iGEM! If you have any questions about using it, please don't hesitate to contact us at hq (at) igem (dot) org

• (3) 96-well skirted PCR plates
• (3) universal plate lids
• (5) adhesive foils
• bubble wrap
• a box with the iGEM HQ address, for shipping your submission

Sample Layout

Make sure to add your samples in the correct layout:

• Your first sample (s1) should be dispensed into well 1A of your submission plate.
• Your second sample (s2) should be dispensed into well 1B of the same submission plate.

Use one plate per a submission order. Example: If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.

Submission DNA Preparation

• 250ng minimum of purified plasmid DNA (your BioBrick compatible part in pSB1C3)
  • 10ul of a 25ng/ul miniprep will work well
• DNA must be dried down before shipping

We recommend drying down the plate in your lab's laminar flow hood (you can also leave your plate on the lab bench to air-dry, covered with the plastic lid).

1. Sterilize the hood surface with 70% Ethanol
2. Cover the 96 well plate with the provided plate lid (not with the adhesive foil)
3. Place the plate in the hood.
4. With sufficient airflow, drying down 10ul of 25ng/ul (250ng total) of purified plasmid DNA in the plate should take 4-6 hours (or overnight).
5. Once all wells have dried, cover the plate wells with the provided adhesive foil.
   1. The adhesive foil should make full contact with the wells of the plate (see picture). When properly sealed you should be able to see the impression of every well.
6. Cover the sealed plate with a plastic lid
7. Label your plate

Plate Labelling

Make sure to label each plate with your group (team/lab) name and the appropriate submission number. Use a lab marker and write your team name and submission number on the side of the 96w plate.

Use one plate per a submission order. Example: If you are submitting 104 parts, create one submission order for 96 parts and another submission order for 8 parts. Label the plates with the correct submission order.

Note: When filling shipment info please make sure to declare and label the package as "DNA (non-hazardous, non-regulated, non-infectious, for research purposes only)."

Packaging your submission for shipping

Place your submission in an appropriately sized shipping container, such as a cardboard box, along with the printout of the submission form. If you use cushioning material inside the container, limit cushioning material to foam peanuts, bubble wrap, packaging paper, or similar.

Note: Part submissions packaged in materials other than the above will be rejected and these part submissions will not be taken into consideration for medals or awards. It is important that you not hide your submissions in an effort to conceal them from customs, because they will be seized.

IMPORTANT: iGEM 2018 Teams in China

For 2018, iGEM HQ and the Registry are collaborating with Genscript to help iGEM Teams in China submit their part samples. In the past, some teams from China have had issues shipping to the United States. With this offer, iGEM Teams in China will ship their part samples to Genscript, and Genscript will forward these shipments to iGEM HQ.

Genscript Shipping Details

• Ship to address: 江苏省南京市江宁区江宁科学园雍熙路28号 南京金斯瑞生物科技 中国业务部物流中心
• Receiver: 舒丹 Shudan
• Tel: 400-025-8686转分机号3908 或 5836
• Email: Dan.shu@genscript.com / Jianfang.cao@genscript.com
• Note: 请您按照IGEM官网说明将kit内容准备完好并于10月10日（before deadline）邮寄至金斯瑞，我们将于截至日次日统一将各团队kit寄出至IGEM。请在包裹中附上您的团队信息（团队名称及联系方式）

Please include all required documents in the package.

All iGEM Teams in China must still follow the normal directions for part submission:

• miniprepped plasmid DNA (over 250ng) dried down. 10ul of 25ng/ul for a total of 250ng works well.
• in 96 well plate format (label your plate with your team name and submission number)
• plate sealed with adhesive foil
  • the adhesive foil should make full contact with the wells of the plate (see picture)
• sealed plate covered with a plastic lid
• shipped with a tracking number
• shipped and labelled as DNA, not hidden or disguised
  • DNA (non-hazardous, non-regulated, non-infectious, for research purposes only)

Archive

Archived Submission Formats Page