Difference between revisions of "Part:BBa K2365037"
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====PCR and SDS-PAGE==== | ====PCR and SDS-PAGE==== | ||
Showing the size of the target gene. | Showing the size of the target gene. | ||
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[[File:Don degrading enzyme 1.jpg|400px]] | [[File:Don degrading enzyme 1.jpg|400px]] | ||
[[File:DON degrading enzyme SDS.jpg|400px]] | [[File:DON degrading enzyme SDS.jpg|400px]] | ||
Revision as of 11:15, 31 October 2017
Trichothecene 3-O-acetyltransferase(AYT1)，an indispensable enzyme for the biosynthesis of trichothecenes, to catalyzes the transfer of an acetyl group from acetyl coenzyme A to the C3 hydroxyl moiety which will result in a switch from DON to 3ADON, so the toxicity will be reduced by 100-fold.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 213
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 306
Illegal BglII site found at 318
Illegal XhoI site found at 884 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
PCR and SDS-PAGE
Showing the size of the target gene.
