Difference between revisions of "Part:BBa K2387045"
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[[file:T--Wageningen_UR--mVenus.jpg|400px|center|thumb|<p align="justify">'''Figure 1: On the left, <i>E. coli</i> BL21 without plasmid. On the right, E. coli BL21 expressing anm2CP induced by 0.2% arabinose.'''</p>]]
 
[[file:T--Wageningen_UR--mVenus.jpg|400px|center|thumb|<p align="justify">'''Figure 1: On the left, <i>E. coli</i> BL21 without plasmid. On the right, E. coli BL21 expressing anm2CP induced by 0.2% arabinose.'''</p>]]
  
−
This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([[BBa_K2387032]]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.  
+
This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([ https://parts.igem.org/Part:BBa_K2387032 BBa_K2387032]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.  
  
 
<table style="width:100%">
 
<table style="width:100%">
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<tr>
 
<tr>
 
<td>mRFP</td>
 
<td>mRFP</td>
−
<td>[[BBa_K2387054]]</td>
+
<td>[https://parts.igem.org/Part:BBa_K2387054 BBa_K2387054]</td>
−
<td>[[BBa_K2387055]]</td>
+
<td>[https://parts.igem.org/Part:BBa_K2387055 BBa_K2387055]</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td>eYFP</td>
 
<td>eYFP</td>
−
<td>[[BBa_K2387003]]</td>
+
<td>[https://parts.igem.org/Part:BBa_K2387003 BBa_K2387003]</td>
−
<td>[[BBa_K2387065]]</td>
+
<td>[https://parts.igem.org/Part:BBa_K2387065 BBa_K2387065]</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td>mVenus</td>
 
<td>mVenus</td>
−
<td>[[BBa_K2387045]]</td>
+
<td>[https://parts.igem.org/Part:BBa_K2387045 BBa_K2387045]</td>
−
<td>[[BBa_K2387046]]</td>
+
<td>[https://parts.igem.org/Part:BBa_K2387046 BBa_K2387046]</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td>sfGFP</td>
 
<td>sfGFP</td>
−
<td>[[BBa_K2387047]]</td>
+
<td>[https://parts.igem.org/Part:BBa_K2387047 BBa_K2387047]</td>
−
<td>[[BBa_K2387048]]</td>
+
<td>[https://parts.igem.org/Part:BBa_K2387048 BBa_K2387048]</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td>mCerulean</td>
 
<td>mCerulean</td>
−
<td>[[BBa_K2387052]]</td>
+
<td>[https://parts.igem.org/Part:BBa_K2387052 BBa_K2387052]</td>
−
<td>[[BBa_K2387053]]</td>
+
<td>[https://parts.igem.org/Part:BBa_K2387053 BBa_K2387053]</td>
 
</tr>
 
</tr>
 
</table>
 
</table>

Revision as of 11:09, 31 October 2017


mVenus controlled by inducible araC/pBAD promoter

mVenus is a bright yellow monomeric fluorescent protein. In this composite, its expression is regulated by the araC/pBad promoter and by a strong RBS. mVenus presents a maximum pick of excitation at 515 nm and a maximum pick of emission at 528. mVenus was engineered from YFP to grant it a faster maturation and to make more resistant to pH and chloride ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Functional Parameters

In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1).

Figure 1: On the left, E. coli BL21 without plasmid. On the right, E. coli BL21 expressing anm2CP induced by 0.2% arabinose.

This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([ https://parts.igem.org/Part:BBa_K2387032 BBa_K2387032]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.

Table 1: Split Proteins.
Protein Part Number (Full length) Part Number (Split Protein)
mRFP BBa_K2387054 BBa_K2387055
eYFP BBa_K2387003 BBa_K2387065
mVenus BBa_K2387045 BBa_K2387046
sfGFP BBa_K2387047 BBa_K2387048
mCerulean BBa_K2387052 BBa_K2387053