Difference between revisions of "Part:BBa K2387045"
Line 33: Line 33:
 
<tr>
 
<tr>
 
<td>mRFP</td>
 
<td>mRFP</td>
−
<td>[BBa_K2387054]</td>
+
<td>[[BBa_K2387054]]</td>
 
<td>[[BBa_K2387055]]</td>
 
<td>[[BBa_K2387055]]</td>
 
</tr>
 
</tr>

Revision as of 11:07, 31 October 2017


mVenus controlled by inducible araC/pBAD promoter

mVenus is a bright yellow monomeric fluorescent protein. In this composite, its expression is regulated by the araC/pBad promoter and by a strong RBS. mVenus presents a maximum pick of excitation at 515 nm and a maximum pick of emission at 528. mVenus was engineered from YFP to grant it a faster maturation and to make more resistant to pH and chloride ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Functional Parameters

In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1).

Figure 1: On the left, E. coli BL21 without plasmid. On the right, E. coli BL21 expressing anm2CP induced by 0.2% arabinose.

This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system (BBa_K2387032). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.

Table 1: Split Proteins.
Protein Part Number (Full length) Part Number (Split Protein)
mRFP BBa_K2387054 BBa_K2387055
eYFP BBa_K2387003 BBa_K2387065
mVenus BBa_K2387045 BBa_K2387046
sfGFP BBa_K2387047 BBa_K2387048
mCerulean BBa_K2387052 BBa_K2387053