Difference between revisions of "Part:BBa K2387045"
Line 20: Line 20:
 
In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1).  
 
In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1).  
  
−
<div class="figure-center">
+
[[file:T--Wageningen UR--mVenus.jpg|400px|center|thumb|<p align="justify">'''Figure 1: On the left, <i>E. coli</i> BL21 without plasmid. On the right, E. coli BL21 expressing anm2CP induced by 0.2% arabinose.'''</p>]]
−
<img class="figure-center-img" src="https://static.igem.org/mediawiki/2017/5/55/T--Wageningen_UR--mVenus.jpg"/>
+
−
<div class="figure-center-caption">
+
−
<b>Figure 1:</b> Absorbance and fluorescence spectra from mVenus measured after induction with 0.002% arabinose.
+
−
</div>
+
−
</div>
+
  
 
This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([[BBa_K2387032]]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.  
 
This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([[BBa_K2387032]]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.  
Line 64: Line 59:
 
</tr>
 
</tr>
 
</table>
 
</table>
−
 
−
</HTML>
 

Revision as of 10:58, 31 October 2017


mVenus controlled by inducible araC/pBAD promoter

mVenus is a bright yellow monomeric fluorescent protein. In this composite, its expression is regulated by the araC/pBad promoter and by a strong RBS. mVenus presents a maximum pick of excitation at 515 nm and a maximum pick of emission at 528. mVenus was engineered from YFP to grant it a faster maturation and to make more resistant to pH and chloride ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Functional Parameters

absMax. 515 nm
chassisE. coli
colorYellow Fluorescence, Pale Yellow cells
emissionMax. 528 nm

In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1). [[file:T--Wageningen UR--mVenus.jpg|400px|center|thumb|

'''Figure 1: On the left, E. coli BL21 without plasmid. On the right, E. coli BL21 expressing anm2CP induced by 0.2% arabinose.'''

]] This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([[BBa_K2387032]]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.
Table 1: Split Proteins.
Protein Part Number (Full length) Part Number (Split Protein)
mRFP [[BBa_K2387054]] [[BBa_K2387055]]
eYFP [[BBa_K2387003]] [[BBa_K2387065]]
mVenus [[BBa_K2387045]] [[BBa_K2387046]]
sfGFP [[BBa_K2387047]] [[BBa_K2387048]]
mCerulean [[BBa_K2387052]] [[BBa_K2387053]]