Difference between revisions of "Part:BBa K2286005"
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This part contains a root-specific promoter from Arabidopsis thaliana root and Hypocotyls. Compared to the full-length promoter, | This part contains a root-specific promoter from Arabidopsis thaliana root and Hypocotyls. Compared to the full-length promoter, | ||
| − | PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times | + | PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times. |
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PYK10-1457 is an excellent root-specific promoter. | PYK10-1457 is an excellent root-specific promoter. | ||
| − | [[File:T--UESTC-China--PYK10-1457-RT-PCR-2.png|800px|thumb|center|'''Fig 1''' | + | [[File:T--UESTC-China--PYK10-1457-RT-PCR-2.png|800px|thumb|center|'''Fig.1''' RT-PCR of CKX3 activated by PYK10-1457.]] |
Revision as of 15:05, 1 November 2017
PYK10-1457：a seedling and root specific promoter
This part contains a root-specific promoter from Arabidopsis thaliana root and Hypocotyls. Compared to the full-length promoter, PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR. The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that PYK10-1457 is an excellent root-specific promoter.
