Difference between revisions of "Part:BBa K2213003"
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<u><font size="+0.5">Assessing the thermal stability of cgPPK2 and cgPPK2-mCherry</font></u>  
 
<u><font size="+0.5">Assessing the thermal stability of cgPPK2 and cgPPK2-mCherry</font></u>  
 
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The heat stability of these constructs was determined through the use of a Thermal Shift Assay of the constructs PPK (BBa_*********) and mCherry-PPK (BBa_*********).
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The heat stability of these constructs was determined through the use of a Thermal Shift Assay of the constructs cgPPK2_His6 (https://parts.igem.org/Part:BBa_K2213003) and PduD(1-20)_cgPPK2_mCherry_His6 (https://parts.igem.org/Part:BBa_K2213004).
  
 
https://static.igem.org/mediawiki/2017/9/9c/Thermalshiftassaymanchesterjess3.png
 
https://static.igem.org/mediawiki/2017/9/9c/Thermalshiftassaymanchesterjess3.png
  
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Firgure 1:
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:46, 1 November 2017


cgPPK2_His6

Cornybacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal


Usage and Biology





Characterisation


Assessing the thermal stability of cgPPK2 and cgPPK2-mCherry
The heat stability of these constructs was determined through the use of a Thermal Shift Assay of the constructs cgPPK2_His6 (https://parts.igem.org/Part:BBa_K2213003) and PduD(1-20)_cgPPK2_mCherry_His6 (https://parts.igem.org/Part:BBa_K2213004).

Thermalshiftassaymanchesterjess3.png

Firgure 1: Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 761