Difference between revisions of "Part:BBa K2377004"

Revision as of 07:02, 31 October 2017

TrxzDP, Thioredoxin Z with two reverse T7 promoters and double terminators to generate dsRNA

This one is initial part in our project. It is made up of Thioredoxin z (TRXz) gene fragment with two reverse T7 promoters and double terminators.

TRX z is a branch of plastidial thioredoxin (TRX), lying between x- and y-type TRXs thus was named TRX z, which is a likely candidate for mediating thiol-modifications in chloroplasts. Recently, several independent studies could demonstrate that TRX z constitutes a subunit of the plastid-encoded RNA polymerase (PEP). Meanwhile the generation of the full PEP complement is an essential early step during chloroplast bio-genesis and its disturbance cannot be reversed if a certain time point in the program has been passed. The Arabidopsis knockout mutant lines of TRX z exhibit a complete albino phenotype and was inhibited in chloroplast development. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
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COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 284
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2377004 short</partinfo>
-This one is initial part in our project. It is made up of Thioredoxin z (TRXz) gene fragment and two reverse T7 promoters. And some research shows the deletion of TRX z expression generates a PEP-deficient phenotype and renders the plants incapable to grow autotrophically and their leaves will turn yellow. Compared to wild type, Phenotypic and transcriptional changes of trx z are obvious.
+This one is initial part in our project. It is made up of Thioredoxin z (TRXz) gene fragment with two reverse T7 promoters and double terminators.
+TRX z is a branch of plastidial thioredoxin (TRX), lying between x- and y-type TRXs thus was named TRX z, which is a likely candidate for mediating thiol-modifications in chloroplasts.
+Recently, several independent studies could demonstrate that TRX z constitutes a subunit of the plastid-encoded RNA polymerase (PEP). Meanwhile the generation of the full PEP complement is an essential early step during chloroplast bio-genesis and its disturbance cannot be reversed if a certain time point in the program has been passed.
+The Arabidopsis knockout mutant lines of TRX z exhibit a complete albino phenotype and was inhibited in chloroplast development.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===