Difference between revisions of "Part:BBa K2440018"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-384 in an Ago2 dependent manner, that is, knockdown of miR-384 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR-384 in an Ago2 dependent manner, that is, knockdown of miR-384 was achieved by transfecting cells with miRNA locker. | ||
| − | Hsa-miR-384 was firstly found in human and rat.1 | + | Hsa-miR-384 was firstly found in human and rat.<sup>1</sup> |
| − | MiR-384 was significantly downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. The overexpression of miR-384 repressed the growth and invasion of NSCLC cells, whereas its suppression showed the opposite effect. Moreover, astrocyte elevated gene-1 (AEG-1) was identified as a target gene of miR-384. The overexpression of miR-384 significantly decreased AEG-1 expression and Wnt signaling, whereas its suppression promoted this pathway. Furthermore, miR-384 was inversely correlated with AEG-1 expression in NSCLC tissues. Additionally, restoration of AEG-1 expression in miR-384-overexpressing cells significantly reversed the antitumor effects of miR- | + | MiR-384 was significantly downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. The overexpression of miR-384 repressed the growth and invasion of NSCLC cells, whereas its suppression showed the opposite effect. Moreover, astrocyte elevated gene-1 (AEG-1) was identified as a target gene of miR-384. The overexpression of miR-384 significantly decreased AEG-1 expression and Wnt signaling, whereas its suppression promoted this pathway. Furthermore, miR-384 was inversely correlated with AEG-1 expression in NSCLC tissues. Additionally, restoration of AEG-1 expression in miR-384-overexpressing cells significantly reversed the antitumor effects of miR-384. <sup>2</sup> |
| − | Moreover, miR-384 suppresses the growth and invasion of Renal Cell Carcinoma Cells (RCC) via downregulation of AEG-1, providing a potential therapeutic target for treatment of | + | Moreover, miR-384 suppresses the growth and invasion of Renal Cell Carcinoma Cells (RCC) via downregulation of AEG-1, providing a potential therapeutic target for treatment of RCC.<sup>3</sup> |
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 00:57, 1 November 2017
miR-384 target sequence
It is the target sequence of miR-384, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-384 in an Ago2 dependent manner, that is, knockdown of miR-384 was achieved by transfecting cells with miRNA locker.
Hsa-miR-384 was firstly found in human and rat.1
MiR-384 was significantly downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. The overexpression of miR-384 repressed the growth and invasion of NSCLC cells, whereas its suppression showed the opposite effect. Moreover, astrocyte elevated gene-1 (AEG-1) was identified as a target gene of miR-384. The overexpression of miR-384 significantly decreased AEG-1 expression and Wnt signaling, whereas its suppression promoted this pathway. Furthermore, miR-384 was inversely correlated with AEG-1 expression in NSCLC tissues. Additionally, restoration of AEG-1 expression in miR-384-overexpressing cells significantly reversed the antitumor effects of miR-384. 2
Moreover, miR-384 suppresses the growth and invasion of Renal Cell Carcinoma Cells (RCC) via downregulation of AEG-1, providing a potential therapeutic target for treatment of RCC.3
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.